MEASUREMENT OF PLATELET ACTIVATION MARKERS CD62P AND CD63 TO DETERMINE THE EFFICIENCY OF DUAL THERAPY WITH ASPIRIN AND CLOPIDOGREL: A PRELIMINARY STUDY

Authors

  • Rachaneekorn Jantasing Faculty of Allied Health Sciences, Thammasat University
  • Ponlapat Rojnuckarin Division of Hematology, Department of Medicine, Faculty of Medicine, Chulalongkorn University
  • Pakrit Jittapiromsak Unit of Neurological Surgery, Department of Surgery, Faculty of Medicine, Chulalongkorn University
  • Wasan Akarathanawat Chulalongkorn Stroke Center, King Chulalongkorn Memorial Hospital, Thai Red Cross Society
  • Dollapak Apipongrat Division of Hematology, Department of Medicine, Phramongkutklao Hospital and College of Medicine
  • Oytip Nathalang Faculty of Allied Health Sciences, Thammasat University

DOI:

https://doi.org/10.55374/jseamed.v8.207

Keywords:

antiplatelet monitoring, drug effectiveness, dual antiplatelet therapy, flow cytometry, platelet aggregation test

Abstract

Background: A dual antiplatelet therapy with aspirin and clopidogrel is advised to prevent stent thrombosis. However, the most appropriate assay for evaluating the antiplatelet effects remains undefined.

Objective: This study aimed to assess the efficacy of measuring platelet surface activation markers, CD62P and CD63, using flow cytometry to determine the effectiveness of dual antiplatelet therapy.

Methods: Thirty patients who received aspirin plus clopidogrel before cerebral artery stent implantation and thirty unrelated healthy controls were enrolled. The expression of CD62P and CD63 was measured using flow cytometry. The diagnostic performances were evaluated and compared with the conventional light-transmitted aggregometry (LTA) using 5.0 and 20.0 µM of adenosine di-phosphate (ADP).

Results: The expression ratios of both markers were significantly lower in the patients receiving dual antiplatelet therapy than controls (p < 0.001). The area under the receiver operating characteristic (ROC) curve (AUC) of CD62P using flow cytometry and the LTA using 5.0 and 20.0 µM of ADP were comparable (0.97 vs. 0.95 and 0.97 vs. 0.96, respectively), whereas those of CD63 was lower than the LTA (0.88 vs. 0.96). The sensitivity of CD62P and CD63 were 71.4% and 60.0%, and the specificity of CD62P and CD63 were 100.0% and 96.7%, respectively.

Conclusion: Flow cytometry measurements of CD62P could be utilized to identify the efficacy of dual antiplatelet therapy. Additional studies are suggested to support this issue and its effects on clinical outcomes.

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References

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Differences in the expression ratios of (A.) CD62P and (B.) CD63 among patients and controls

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Published

2024-05-24

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Jantasing R, Rojnuckarin P, Jittapiromsak P, Akarathanawat W, Apipongrat D, Nathalang O. MEASUREMENT OF PLATELET ACTIVATION MARKERS CD62P AND CD63 TO DETERMINE THE EFFICIENCY OF DUAL THERAPY WITH ASPIRIN AND CLOPIDOGREL: A PRELIMINARY STUDY. J Southeast Asian Med Res [Internet]. 2024 May 24 [cited 2024 Jun. 16];8:e0207. Available from: https://jseamed.org/index.php/jseamed/article/view/207

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